Yes.

You will however, have to remove or negate his culture spreading devices. And if you fail to sieze the planet after it rebels, he will just re-settle it later. What I'm saying is: you will still have to take some military and economic action, but yes, culture can be your most potent weapon.

Sometimes you may have to place capital ships in enemy orbits, even if you do not engage with them, they help repel and spread culture.

No single approach works in SoaSE, you have to combine everything at some level. There is one thing I am uncertain of, however. I am not sure if you can cause a homeworld to rebel via culture, it's possible that you will have to claim the last system militarily. Every other world with certainty, however, can fall to culture.

Flip enough worlds and the AI may actually simply surrender.

Wait, the DE actually worked?

That's...

...IMPOSSIBLE!

j/k.

If Timed Explosives actually worked (is it just me or do they never go off?) and did enough damage, I'd say that waves of suicide Arcova Scouts might do the trick by sniping out their culture centers if you were TEC.

On the other hand you could try using the Seeker's suicide attack, which would be even more ironic.

Don't knock it...

Watch 10 Seeker Vessels 'Martyr' themselves against a high level capital ship that just got it's shields knocked down.

For 2000 credits spent, you get get a nice guarantee that a vessel that cost 3,000 creds, + experience gain (or purchase!), and bucket full of resources, will die...

You come out quite a bit ahead of the game for the loss of some crazy jihadis and pocket change.